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. 2003 Mar;185(5):1616–1623. doi: 10.1128/JB.185.5.1616-1623.2003

FIG. 1.

FIG. 1.

Schematic diagram of the F-like plasmid transfer region. (A) F-plasmid tra operon: schematic diagram of the first 2,700 bp and the last 600 bp of the 33.3-kb tra operon (11). The positions of the origin of transfer (oriT), the traM, traJ, traY, traA, and finO genes, and the finP antisense RNA are indicated. Promoters are indicated by ellipses. (B) Genetic organization of the traJ promoter region of the F-like plasmid pRK100. The positions of the −35 and −10 promoter regions, transcription initiation (traJ mRNA and finP RNA) and termination (finP RNA termination), the ribosomal binding site (RBS), and initiation of TraJ translation (TraJ) are indicated. The location of upstream element (UP) from position −40 to position −60 is also shown. The binding sites of oligonucleotide primers PtraJ-1 and PtraJ-2 are indicated by solid arrows. The sequence of the pRK100 plasmid differs at one base (indicated by an asterisk) from the published sequence of the same region of the F plasmid (GenBank accession number U01159).