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. 2003 Mar;185(5):1616–1623. doi: 10.1128/JB.185.5.1616-1623.2003

FIG. 2.

FIG. 2.

β-Galactosidase (β-gal) activity of the traJ-lacZ fusion. At various times (0 to 8 h), the optical densities at 600 nm (OD 600 nm) of the cultures were measured (dotted lines), and aliquots were assayed for β-galactosidase activity (expressed in Miller units [MU])(solid lines). (A) β-Galactosidase activities of the traJ-lacZ fusion in wild-type strain MC4100 grown in LB medium (▴) and in conditioned LB medium (•). (B) β-Galactosidase activities of the traJ-lacZ fusion in wild-type strain MC4100 grown in M63 medium with 1× glucose (•) and with 0.2× glucose (▴). (C) β-Galactosidase activities of the traJ-lacZ fusion in wild-type (w.t.) strain MC4100 (▴) and in the MC4100 cya mutant (×) and crp mutant (•) strains grown in LB medium. (D) β-Galactosidase activities of the traJ-lacZ fusion in wild-type strain MC4100 grown in LB medium (▴) and in the MC4100 cya mutant strain grown in LB medium with (⧫) and without (×) 10 mM cAMP. (E) β-Galactosidase activities in strain MC4100 carrying the wild-type traJ-lacZ fusion (w.t. PtraJ) (▴) or the traJ-lacZ fusion in which the left (ML PtraJ) (◊) or right (MR PtraJ) (○) part of the CRP binding domain was altered. Experiments were carried out three or four times, and representative results are shown.