TABLE 3.
Electron donor specificity of T. kodakaraensis hydrogenase (H2 evolution)
| Electron donora | E°′ (mV) | Concn (mM) | Sp act (U/mg) |
|---|---|---|---|
| Methylene blue | +11 | 0.1 | 0 |
| FAD | −219 | 0.3 | 0 |
| FMN | −219 | 0.3 | 0 |
| NADH | −320 | 1 | 0 |
| NADPH | −320 | 1 | 2.72 |
| NADP+ + GDH + l-Glu | −320 | 0.01 | 4.12 |
| Benzyl viologen | −360 | 1 | 87.3 |
| Methyl viologen | −446 | 1 | 283 |
a
GDH, glutamate dehydrogenase of T. kodakaraensis KOD1 (1.7 μg); l-Glu, l-glutamate (final, 10 mM); sodium dithionite (final, 20 mM) was added as a reducing reagent except for NADH, NADPH, and NADP+ + glutamate dehydrogenase + l-glutamate. Also see Table 2, footnote a.