FIG. 3.
Two-dimensional gel analysis of total proteins isolated from stationary-phase M. smegmatis. (A) Wild-type strain mc2155 grown under oxygen-starvation conditions. (B) devR mutant OTL1 grown under oxygen starvation conditions. (C) Wild-type strain mc2155 grown under carbon starvation conditions. The positions of five DevR-dependent proteins are illustrated in each gel, and their identities were determined by N-terminal sequencing. Spot 1, UspL; spot 2, UspM, spot 3, UspN; spot 4, putative nitroreductase; spot 5, HspX. UspL, UspM, and UspN are members of the USP family. The nitroreductase shows strong similarity to Rv2032, a proposed nitroreductase in M. tuberculosis. HspX is the M. smegmatis homologue of the α-crystallin (HspX) protein of M. tuberculosis. Gels shown are representative of 3 independent experiments.