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. 2000 Feb;11(2):735–746. doi: 10.1091/mbc.11.2.735

Figure 4.

Figure 4

IRS-1 and IRS-2 but not IRS-3 suppress apoptotic cell death. (A) IRS-1, 4F-IRS-1 (4F), and IRS-2 but not IRS-3 associate with Bcl-2 in Ba/F3 cells. Lysates from the indicated cell lines were immunoprecipitated and immunoblotted with the indicated antibodies. P-Bcl-2, phosphorylated Bcl-2. (B) Upper panel, lysates from the indicated cell lines were immunoblotted with anti-insulin receptor β chain (αIRβ). Lower panel, lysates from the indicated cell lines were immunoprecipitated and immunoblotted with indicated antibodies. (C) IRS-1 and IRS-2 but not IRS-3 suppress apoptosis of Ba/F3 cells deprived of IL-3. Stable transfectants of IRS-1, IRS-2, IRS-3, 4F-IRS-1, and Bcl-2 were deprived of IL-3 and cultured in RPMI 1640 medium containing 5% FCS. FCS used in this study contained 15 nM insulin quantified by IRMA. The FACS analysis was performed 48 h after IL-3 depletion.