TABLE 3.

Sequential and simultaneous reactions against plant cell wall by recombinant cellulosomes of the cellulase mixture and of XynA^a

Added cellulosome(s)		Mean amt (μmol/ml) of liberated reducing sugarb ± SD	Degree of synergyc
First reaction	Second reaction
Simultaneous reaction
Cellulase + XynA	No enzyme	0.622 ± 0.014	1.61
No enzyme	Cellulase + XynA	0.595 ± 0.043	1.59
Sequential reaction
Cellulase	XynA	0.407 ± 0.022	1.03
XynA	Cellulase	0.357 ± 0.048	0.98
Control reaction
Cellulase	No enzyme	0.300 ± 0.003
No enzymes	Cellulase	0.278 ± 0.010
XynA	No enzyme	0.087 ± 0.004
No enzyme	XynA	0.096 ± 0.002
No enzyme	No enzyme	0.007 ± 0.003

^aCorn cell walls were degraded by 0.5 nmol of XynA cellulosome (XynA), 1 nmol of cellulase cellulosomes (cellulase), or 1 nmol of cellulase cellulosome plus 0.5 nmol of XynA cellulosome (XynA + cellulases)/ml at 37°C for 15 h. Between the first and the second reactions, the reaction mixtures were boiled for 20 min to inactivate the cellulosomes used for the first reaction.

^bThe values are the average of three determinations.

^cThe synergy degree values were calculated as the activity divided by the sum of the activities of the control reactions.