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Sensitivity of wild-type and mutant CheY proteins to dephosphorylation by CheZ. The rates of release of Pi in mixtures containing CheY (4.5 μM), phosphoimidazole (3 mM), and various amounts of CheZ were measured spectroscopically with the Enzchek Pi assay kit (Molecular Probes). Data are shown for wild-type CheY (squares), CheYE89Q (triangles), and CheYE89A (diamonds). The lines were generated by least-squares fitting, and the slopes were used as an indicator of CheZ sensitivity.
