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. 2003 Mar;185(5):1659–1671. doi: 10.1128/JB.185.5.1659-1671.2003

FIG. 3.

FIG. 3.

Tricine-SDS-PAGE gel showing the migration of LPS from E. coli CWG345 (waaZ::aphA-3) and the effects of WaaZ overexpression on LPS mobility in strains representing different core types. LPS samples were separated on a 10 to 20% Tricine gel by SDS-PAGE and visualized by silver staining. All strains produce R-LPS, therefore only the region of the gel containing the lipid A core species is shown. (A) LPS mobility of CWG345 (waaZ) with (+) and without (−) plasmid-borne waaZ (pWQ152) compared to the wild-type strain W3100. The arrow indicates a faster-migrating species resulting from overexpression of WaaZ. (B) The effect on LPS migration of adding multicopy WaaZ to the representative prototype strains for the E. coli core types. Expression from pWQ152 was induced by the addition of 0.02% l-arabinose. − and +, uninduced and induced cultures, respectively.