TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype, serotype, or description | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | K-12 φ80d deoR lacZΔM15 endA1 recA1 hsdR17(rK− mK+) supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 F− | 34 |
| BL21(λDE3) | F−ompT hsdSB(rB− mB−) gal dcm (λDE3) | Novagen |
| W3100 | E. coli K-12 core OS prototype R-LPS, IN(rrnD-rrnE)1 | CGSC4466a |
| F470 | E. coli R1 core OS prototype, R-LPS derivative of O8:K27 | 19, 37, 47 |
| F632 | E. coli R2 core OS prototype, R-LPS derivative of O100:K? (B):H2 | 17, 18 |
| F653 | E. coli R3 core OS prototype, R-LPS derivative of O111:K58− | 35 |
| F2513 | E. coli R4 core OS prototype, R-LPS derivative of O14:K7 | 19, 36 |
| CWG28 | trp his lac rpsL cpsK30-1 derivative of strain E69 (O9:K−) | 45 |
| CWG296 | waaP::aacC1 derivative of F470, Gmr | 47 |
| CWG297 | waaQ::aacC1 derivative of F470, Gmr | 47 |
| CWG303 | waaG::aacC1 derivative of F470, Gmr | 19 |
| CWG308 | waaO::aacC1 derivative of F470, Gmr | 19 |
| CWG309 | waaT::aacC1 derivative of F470, Gmr | 19 |
| CWG310 | waaW::aacC1 derivative of F470, Gmr | 19 |
| CWG311 | waaV::aacC1 derivative of F470, Gmr | 19 |
| CWG312 | waaY::aacC1 derivative of F470, Gmr | 47 |
| CWG345 | waaZ::aphA-3 derivative of W3100, Kmr | This study |
| S. enterica serovar Typhimurium LT2 SL3770 | waa+, S-LPS | SGSC225b |
| Plasmids | ||
| pBAD18-Ap | Arabinose-inducible expression vector, Apr | 15 |
| pBAD24 | Arabinose-inducible expression vector, Apr | 15 |
| pET28a(+) | IPTG-inducible expression vector, Kmr | Novagen |
| pYA3265 | Source of the nonpolar aphA-3 cassette conferring kanamycin resistance, Kmr | A. Honeyman via E. Vimr |
| pMAK705 | pUC19-pMAK700 hybrid suicide vector containing the temperature-sensitive pSC101 replicon, Cmr | 16 |
| pWQ152 | pBAD24-derivative carrying the waaZ coding region from W3100 as an NcoI-HindIII fragment, Apr | This study |
| pWQ153 | pET28a(+) derivative carrying the EcoRI-HindIII fragment containing the waaZ coding region from pWQ152 | This study |
| pWQ154 | pBAD18-Ap derivative containing an XbaI-HindIII fragment from pWQ153 and expressing the His6-tagged WaaZ gene product | This study |
| pWQ160 | pBAD24 derivative carrying the waaF coding region from W3100 as an NcoI-HindIII fragment, Apr | This study |
a
E. coli Genetic Stock Center.
b
Salmonella Genetic Stock Center.