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. 2003 Mar;185(5):1739–1744. doi: 10.1128/JB.185.5.1739-1744.2003

FIG. 4.

FIG. 4.

Western blot analysis of B. henselae whole-cell protein and recombinant Pap31 in E. coli M15(pREP4) with monoclonal Pap31 antibody VKS29. (A) Outer membrane proteins. M, prestained molecular mass standard (Bio-Rad Laboratories); lane 1, E. coli M15(pREP4); lane 2, E. coli M15(pREP4) with pQE70; lane 3, E. coli M15 pap31 before induction; lane 4, E. coli M15 pap31 after induction. The arrow indicates the recombinant Pap31. (B) Bartonella henselae whole-cell protein sample immunoblots incubated with monoclonal antibody VKS29 (lane 1) and polyclonal immunserum (lane 3). Lane 2, negative control with secondary antibody and without primary antibody (horseradish peroxidase-conjugated goat anti-mouse immunoglobulin G antibody).