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. 2003 Mar;185(5):1739–1744. doi: 10.1128/JB.185.5.1739-1744.2003

FIG. 5.

FIG. 5.

Two-dimensional analysis of the recombinant Pap31 with the outer membrane proteins from E. coli M15 pap31 before and after induction. Pap31 has an calculated pI of 5.2 and was detected with the monoclonal Pap31 antibody VKS29. Shown are outer membrane proteins from E. coli M15 pap31 before (A) and after (B) induction. The arrow points to the recombinant Pap31 at the pI of 5. A molecular mass standard (Bio-Rad Laboratories) is shown at the left side of each panel.