TABLE 1.
Strain or plasmid | Relevant characteristic(s) | Source or reference |
---|---|---|
Strains | ||
Bartonella henselae | Type strain | ATCC 49882 |
E. coli K-12 EB53 | aroB tsx malT hemA | Generous gift of Klaus Hantke, Tübingen, Germany |
E. coli M15(pREP4) | Host for expression vectors pQE70 and pQE60 | Qiagen |
E. coli M15 pap31 | As E. coli M15(pREP4) pRZn7-Pap31(ompT) | This study |
E. coli EB53(pREP4) | As E. coli K-12 EB53 KmrlacI p15A ori | This study |
E. coli EB53 pap31 | As E. coli EB53(pREP4) pRZn7-Pap31(ompT) Kmr | This study |
Plasmids | ||
pQE60 | Expression vector; ColE1 PT5 lacO Ampr | Qiagen |
pQE70 | Expression vector; ColE1 PT5 lacO Ampr | Qiagen |
pRZn7-Pap31 (ompT) | As pQE70 pap31(ompT) | This study |
pREP4 | lacIq Kmr p14A ori | Qiagen |