TABLE 1.
B. subtilis strains and plasmids used
| Plasmid or strain | Relevant characteristicsa | Origin or reference |
|---|---|---|
| Plasmids | ||
| pDH1 | pBluescript SK(−) spoIIE | This study |
| pDH2 | erm @ HincII site of pDH1 upstream of spoIIE ORF | This study |
| pDH3 | spc @ HincII site of pDH1 upstream of spoIIE ORF | This study |
| pDH4 | spoIIEV697A in pDH2 background isolated from SL8978 | This study |
| pDH5 | spoIIEV697A inserted by site-directed mutagenesis in pDH3 | This study |
| pDH6 | spoIIQ-gfp neo | This study |
| pDH7 | spoIIEV697A neo | This study |
| pEIA99 | amyE::spoIIQ-lacZ | Edward Amaya |
| pVK59 | Integrative vector with neo | Vasant Chary |
| pVK141 | thrC::spoIIQ-lacZ | Vasant Chary |
| PVK228 | spoIIAC::neo | Vasant Chary |
| Strains | ||
| BR151 | trpC2 metB10 lys-3 | Laboratory stock |
| SL1102 | trpC2 metB10 spoIIAC561 | Laboratory stock |
| SL8410 | trpC2 metB10 lys-3 spoIIGB::erm | This study |
| SL8603 | trpC2 metB10 lys-3 erm spoIIE amyE::spoIIQ-lacZ | This study |
| SL8625 | trpC2 metB10 lys-3 erm spoIIE::pSG1902 amyE::spoIIQ-lacZ | This study |
| SL8675 | trpC2 metB10 lys-3 spc spoIIE::pSG1902 amyE::spoIIQ-lacZ | This study |
| SL8978 | trpC2 metB10 lys-3 erm spoIIEV697A::pSG1902 amyE::spoIIQ-lacZ | This study |
| SL9055 | trpC2 metB10 spoIIAΔ4 | Laboratory stock |
| SL10002 | trpC2 metB10 lys-3 thrC::spoIIQ-lacZ | This study |
| SL10008 | trpC2 metB10 lys-3 erm spoIIEV697A thrC::spoIIQ-lacZ | This study |
| SL10174 | trpC2 metB10 lys-3 amyE::spoIIQ-lacZ | This study |
| SL10221 | trpC2 metB10 lys-3 spoIIQ::pDH6 | This study |
| SL10222 | trpC2 metB10 lys-3 erm spoIIEV697A spoIIQ::pDH6 | This study |
| SL10312 | trpC2 metB10 lys-3 erm spoIIE div-355 amyE::spoIIQ-lacZ | This study |
| SL10339 | trpC2 metB10 lys-3 erm spoIIEV697A amyE::spoIIQ-lacZ | This study |
| SL10423 | trpC2 metB10 lys-3 erm spoIIEV697A div-355 amyE::spoIIQ-lacZ | This study |
| SL10766 | trpC2 metB10 lys-3 erm spoIIEV697A | This study |
| SL10767 | trpC2 metB10 lys-3 erm spoIIE48 | This study |
| SL10905 | trpC2 metB10 spc spoIIEV697A spoIIAΔ4 | This study |
| SL10912 | trpC2 metB10 lys-3 spc spoIIEV697A | This study |
| SL11132 | trpC2 metB10 lys-3 spc spoIIEV697A spoIIGB::erm | This study |
| SL11142 | trpC2 metB10 spc spoIIEV697A spoIIAC561 | This study |
| SL11173 | trpC2 metB10 lys-3 spoIIE::pDH7 | This study |
| SL11201 | trpC2 metB10 lys-3 erm spoIIE48::pDH7 | This study |
| SL11274 | trpC2 metB10 lys-3 spoIIAC::neo | This study |
| SL11275 | trpC2 metB10 lys-3 spc spoIIEV697A spoIIAC::neo | This study |
a
ORF, open reading frame.