TABLE 2.
Pattern of spoIIQ-gfp expression in the parent strain and spoIIEV697A mutant
| Strain | No. of cells showing indicated fluorescence patterna:
|
% Compartment- alizationb | ||
|---|---|---|---|---|
| Forespore | Whole cell | No signal | ||
| SL10221 (parent) | 102 | 4 | 219 | 96 |
| SL10222 (mutant) | 4 | 178 | 145 | 2.2 |
a
These values are the sum of three independent experiments in which cultures were grown in MSSM at 33.5°C and samples were taken at T6. At least 100 cells were scored in each experiment.
b
The number of cells expressing GFP in the forespore was determined as a percentage of the total number of cells expressing GFP.