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. 2003 Mar;185(5):1555–1563. doi: 10.1128/JB.185.5.1555-1563.2003

FIG. 5.

FIG. 5.

Analysis of spore extract from strains 7702, 9602R, RA3R, and 7611R and their mutants. Proteins were separated by SDS-7% PAGE and analyzed by immunoblotting with anti-BclA monoclonal antibody. Lane A, parental strain 7702; lane B, parental strain 9602R; lane C, strain 7702 ΔbclA (PF09); lane D, strain 7702 ΔbclA eag::bclA7702 (PF31); lane E, strain 7702 ΔbclA eag::bclA9602 (PF34); lane F, strain 9602R ΔbclA (PF11); lane G, strain 9602R ΔbclA eag::bclA7702 (PF32); lane H, strain 9602R ΔbclA eag::bclA9602 (PF33); lane I, parental strain RA3R; lane J, strain RA3R ΔbclA (PF13); lane K, strain RA3R ΔbclA eag::bclA7702 (PF35); lane L, strain RA3R ΔbclA eag::bclA9602 (PF36); lane M, parental strain 7611R; lane N, strain 7611R ΔbclA (PF14); lane O, strain 7611R ΔbclA eag::bclA7702 (PF37); lane P, strain 7611R ΔbclA eag::bclA9602 (PF38). The sizes of the molecular mass markers are given in kilodaltons in the left margin.