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Silver-stained polyacrylamide gel (A) and Western immunoblot of LPS reacted with K. pneumoniae O12-specific antiserum (B). Antiserum was obtained and assayed as described in Materials and Methods. LPS samples were prepared by the method of Darveau and Hancock (3). Lane 1, K. pneumoniae KT776; lane 2, E. coli DH5α; lane 3, DH5α harboring pCosKT12; lane 4, cured strain from lane 3; lane 5, DH5α harboring pJTO12; lane 6, E. coli CLM4 (with wb deleted [13]); lane 7, CLM4 harboring pJTO12.
