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. 2003 Mar;185(5):1634–1641. doi: 10.1128/JB.185.5.1634-1641.2003

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FIG. 2. — Silver-stained polyacrylamide gel (A) and Western immunblot of LPS reacted with K. pneumoniae O12-specific antiserum (B). Antiserum was obtained and assayed as described in Materials and Methods. LPS samples were prepared by the method of Darveau and Hancock (3) from K. pneumoniae strains. Lane 1, KT776 (wild type); lane 2, mutant KT776-1 (wzm-wzt); lane 3, mutant KT776-2 (wbbB); lane 4, KT776-1 harboring pST1 (wzm-wzt of wb_KpO12); lane 5, KT776-1 harboring pBR1 (wbbL of wb_KpO12); lane 6, KT776-1 harboring pBR2 (wbbB of wb_KpO12); lane 7, KT776-2 harboring pBR2 (wbbB of wb_KpO12); lane 8, KT776-2 harboring pBR1 (wbbL of wb_KpO12); lane 9, KT776-2 harboring pST1 (wzm-wzt of wb_KpO12).