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. 2000 Feb;11(2):773–793. doi: 10.1091/mbc.11.2.773

Figure 1.

Figure 1

Suppression of cdc24ts and cdc42ts mutations by multicopy MSB1 or multicopy truncated CLA4. The transformants described below were streaked onto SC-Leu-Ura plates with 1 M sorbitol (A) or SC-Leu plates with 1 M sorbitol (B) and incubated at the indicated temperatures. (A) cdc24-11 strain YEF319 was transformed with (1) YEp352-CDC42 and YEp13-CLA4*, (2) YEp352-CDC42 and YEp13-MSB1, (3) YEp13-CLA4* and pPB191 (high-copy MSB1), (4) YEp352-CDC42 and YEp13, (5) YEp24 and YEp13-CLA4*, or (6) YEp24 and YEp13-MSB1. (B) cdc24-12 strain YEF323 (1–3) and cdc42-1 strain DJTD2–16D (4–6) were transformed with (3 and 6) YEp13, (2 and 5) YEp13-CLA4*, or (1 and 4) YEp13-MSB1. (C) Structures of full-length Cla4p and of the truncated Cla4p encoded by plasmid YEp13-CLA4*.