Figure 2.

Suppression of cdc24^ts and cdc42^ts mutations by multicopy MSB3. (A) Physical maps of the inserts in plasmid YEp13-MSBX and in the subclones tested for suppression. All HindIII (H), SacI (S), BglII (G), and BamHI (B) sites are shown. (B and C) The transformants described below were streaked onto SC-Leu-Ura plates with 1 M sorbitol (B) or SC-Leu plates with 1 M sorbitol (C) and incubated at the indicated temperatures. (B) cdc24-11 strain YEF319 harboring plasmid YEp352-CDC42 was transformed with (1) YEp13, (2) YEplac-MSB3D, (3) YEplac-MSB3, or (4) YEplac-ORF1/2. (C) cdc24-13 strain YEF327 (1 and 2), cdc24-12 strain YEF323 (3 and 4), and cdc42-1 strain DJTD2–16D (5 and 6) were transformed with (2, 4, and 6) YEp13 or (1, 3, and 5) YEp13-MSBX.