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. 2000 Feb;11(2):773–793. doi: 10.1091/mbc.11.2.773

Figure 8.

Figure 8

Immunolocalization of HA-tagged Msb4p (A–C) and dependence of Msb3p localization on Cdc42p (D–I). (A–C) Cells of strain YEF1475 containing plasmid YEplac-3HA-MSB4 growing exponentially in SC-Leu liquid medium at 23°C were triple stained for HA-tagged Msb4p (A), actin (B), and DNA (C). Individual cells are numbered for reference in the text. (D–I) Cells of strain YEF1517 (temperature-sensitive for Cdc42p function; see text) harboring plasmid YEplac-3HA-MSB3 growing exponentially in SC-Leu liquid medium at 23°C (D–F) or shifted to 37°C for 4 h (G–I) were triple stained for HA-tagged Msb3p (D and G), actin (E and H), and DNA (F and I).