Table 1.
Saccharomyces cerevisiae strains used in this study
| Strain | Genotype | Reference or source |
|---|---|---|
| YEF473 | a/α his3/his3 leu2/leu2 lys2/lys2 trp1/trp1 ura3/ura3 | Bi and Pringle, 1996 |
| YEF473A | a his3 leu2 lys2 trp1 ura3 | Segregant from YEF473 |
| YEF473B | α his3 leu2 lys2 trp1 ura3 | Segregant from YEF473 |
| YEF313 | a ade2 his3 leu2 lys2 trp1 ura3 cdc24-4 | This studya |
| YEF316 | a ade2 his3 leu2 lys2 trp1 ura3 cdc24-10 | Bi and Pringle, 1996 |
| YEF319 | α ade2 his3 his4 leu2 lys2 trp1 ura3 cdc24-11 | This studya |
| YEF323 | α ade2 his4 leu2 trp1 ura3 cdc24-12 | Bi and Pringle, 1996 |
| YEF327 | α ade2 his4 leu2 trp1 ura3 cdc24-13 | Bi and Pringle, 1996 |
| DJTD2-16D | α gal2 his4 leu2 trp1 ura3 cdc42-1 | Johnson and Pringle, 1990 |
| YEF1234 | as YEF473 except MSB3/msb3Δ∷HIS3 | See text |
| YEF1239 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 | Segregant from YEF1234 |
| YEF1242 | α his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 | Segregant from YEF1234 |
| YEF1292 | as YEF473 except MSB3/msb3Δ∷TRP1 | See text |
| YEF1303 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷TRP1 | Segregant from YEF1292 |
| YEF1235 | as YEF473 except MSB4/msb4Δ∷HIS3 | See text |
| YEF1247 | α his3 leu2 lys2 trp1 ura3 msb4Δ∷HIS3 | Segregant from YEF1235 |
| YEF1249 | a his3 leu2 lys2 trp1 ura3 msb4Δ∷HIS3 | Segregant from YEF1235 |
| YEF1236 | as YEF473 except MSB4/msb4Δ∷TRP1 | See text |
| YEF1256 | a his3 leu2 lys2 trp1 ura3 msb4Δ∷TRP1 | Segregant from YEF1236 |
| YEF1258 | α his3 leu2 lys2 trp1 ura3 msb4Δ∷TRP1 | Segregant from YEF1236 |
| YEF1293 | as YEF473 except MDR1/mdr1Δ∷HIS3 | See text |
| YEF1310 | a his3 leu2 lys2 trp1 ura3 mdr1Δ∷HIS3 | Segregant from YEF1293 |
| YEF1312 | α his3 leu2 lys2 trp1 ura3 mdr1Δ∷HIS3 | Segregant from YEF1293 |
| YEF1294 | as YEF473 except YPL249C/ypl249CΔ∷HIS3 | See text |
| YEF1321 | α his3 leu2 lys2 trp1 ura3 ypl249CΔ∷HIS3 | Segregant from YEF1294 |
| YEF1295 | as YEF473 except YPL249C/ypl249CΔ∷TRP1 | See text |
| YEF1329 | a his3 leu2 lys2 trp1 ura3 ypl249CΔ∷TRP1 | Segregant from YEF1295 |
| YEF1330 | α his3 leu2 lys2 trp1 ura3 ypl249CΔ∷TRP1 | Segregant from YEF1295 |
| YEF1269 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷HIS3 | Segregant from YEF1239 × YEF1247bc |
| YEF1270 | α his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷HIS3 | Segregant from YEF1239 × YEF1247b |
| YEF1289 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷TRP1 | Segregant from YEF1239 × YEF1258 |
| YEF1291 | α his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷TRP1 | Segregant from YEF1239 × YEF1258 |
| YEF1631 | as YEF473 except msb3Δ∷HIS3/msb3Δ∷HIS3 msb4Δ∷TRP1/msb4Δ∷TRP1 | YEF1289 × YEF1291d |
| YEF1358 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷TRP1 mdr1Δ∷HIS3 | Segregant from YEF1303 × YEF1312 |
| YEF1360 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 ypl249CΔ∷TRP1 | Segregant from YEF1239 × YEF1330 |
| YEF1362 | a his3 leu2 lys2 trp1 ura3 msb4Δ∷TRP1 mdr1Δ∷HIS3 | Segregant from YEF1256 × YEF1312 |
| YEF1363 | α his3 leu2 lys2 trp1 ura3 msb4Δ∷TRP1 mdr1Δ∷HIS3 | Segregant from YEF1256 × YEF1312 |
| YEF1364 | a his3 leu2 lys2 trp1 ura3 msb4Δ∷HIS3 ypl249CΔ∷TRP1 | Segregant from YEF1249 × YEF1330 |
| YEF1366 | a his3 leu2 lys2 trp1 ura3 mdr1Δ∷HIS3 ypl249CΔ∷TRP1 | Segregant from YEF1310 × YEF1330 |
| YEF1354 | α his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷TRP1 mdr1Δ∷HIS3 | Segregant from YEF1291 × YEF1310b |
| YEF1356 | a his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷HIS3 ypl249CΔ∷TRP1 | Segregant from YEF1270 × YEF1329b |
| YEF1389 | α his3 leu2 lys2 trp1 ura3 msb3Δ∷TRP1 mdr1Δ∷HIS3 ypl249CΔ∷HIS3 | Segregant from YEF1358 × YEF1321b |
| YEF1390 | a his3 leu2 lys2 trp1 ura3 msb4Δ∷TRP1 mdr1Δ∷HIS3 ypl249CΔ∷HIS3 | Segregant from YEF1362 × YEF1321b |
| YEF1393 | α his3 leu2 lys2 trp1 ura3 msb3Δ∷HIS3 msb4Δ∷TRP1 mdr1Δ∷HIS3 ypl249CΔ∷TRP1 | Segregant from YEF1360 × YEF1363bc |
| YEF1474 | as YEF473 except msb3Δ∷HIS3/msb3Δ∷HIS3 ura3:URA3:3HA-MSB3/ura3:URA3:3HA-MSB3 | See text |
| YEF1475 | as YEF473 except msb4Δ∷HIS3/msb4Δ∷HIS3 ura3:URA3:3HA-MSB4/ura3:URA3:3HA-MSB4 | See text |
| YEF1517 | a his3 leu2 lys2 trp1 ura3 cdc42Δ∷HIS3 ura3:URA3:GFP-CDC42 | See text |
| JF16 | a ade2 his3 leu2 lys2 trp1 ura3 bni1Δ∷LEU2 | H. Fares and J.R. Pringlee |
| YJZ426 | as YEF473A except bni1Δ∷HIS3 | J. Zahner and J.R. Pringlef |
| HH799 | as YEF473 except bni1Δ∷HIS3/bni1Δ∷HIS3 | J. Zahner and J.R. Pringlef |
| CCY1042-12B | α his3 leu2 lys2 trp1 ura3 gic1-Δ1∷LEU2 gic2-Δ2∷TRP1 | This studyg |
| CCY1024-19C | α his3 leu2 trp1 ura3 gic1-Δ1∷LEU2 gic2-1∷HIS3 | Chen et al., 1997a |
| YEF1662 | a/α his3/his3 leu2/leu2 lys2/lys2 trp1/trp1 ura3/ura3 gic1-Δ1∷LEU2/gic1-Δ1∷LEU2 gic2-Δ2∷TRP1/gic2-Δ2∷TRP1 | This studyh |
| YEF933 | as YEF473 except BEM3/bem3Δ∷HIS3 | See text |
| YEF992 | a his3 leu2 lys2 trp1 ura3 bem3Δ∷HIS3 | Segregant from YEF933 |
| YEF959 | as YEF473 except RGA1/rga1Δ∷HIS3 | See text |
| YEF1000 | α his3 leu2 lys2 trp1 ura3 rga1Δ∷HIS3 | Segregant from YEF959 |
| YEF1203 | α his3 leu2 lys2 trp1 ura3 bem3Δ∷HIS3 rga1Δ∷HIS3 | Segregant from YEF992 × YEF1000c |
| YEF1206 | a his3 leu2 lys2 trp1 ura3 bem3Δ∷HIS3 rga1Δ∷HIS3 | Segregant from YEF992 × YEF1000c |
| YEF1223 | as YEF473 except bem3Δ∷HIS3/bem3Δ∷HIS3 rga1Δ∷HIS3/rga1Δ∷HIS3 | YEF1203 × YEF1206 |
| YEF1265 | as YEF473 except bem3Δ∷HIS3/bem3Δ∷HIS3 rga1Δ∷HIS3/rga1Δ∷HIS3 CDC24/cdc24Δ∷TRP1 | See text |
| YEF1154 | as YEF473 except CDC24/cdc24Δ∷HIS3 | See text |
| YEF1201 | a his3 leu2 lys2 trp1 ura3 cdc24Δ∷HIS3 [pMGF5] | This studyi |
Derived by several crosses from the original cdc24 mutant strains isolated in the screens described by Sloat et al. (1981) and Harris and Pringle (1991).
The genotypes of these strains were inferred from the 2 His+:2 His− (and, for YEF1393, 2 Trp+:2 Trp−) segregation pattern in the appropriate tetrad.
The genotypes of these strains were confirmed by outcrossing them to strain YEF473A or YEF473B.
Constructed by crossing strain YEF1289 harboring plasmid YEplac-MSB3 to strain YEF1291 harboring plasmid YEp352, selecting diploids on SC−Leu−Ura plates, and then curing both plasmids.
Strain YPH501 (Sikorski and Hieter, 1989) was transformed with a DNA fragment in which nearly all of the BNI1 open reading frame had been replaced by LEU2; JF16 is a segregant from such a transformant.
bni1Δ∷HIS3 is a complete deletion of the BNI1 open reading frame constructed using the method of Baudin et al. (1993). Strain HH799 was constructed by mating YJZ426 and an isogenic α strain.
Derived from strains described by Chen et al. (1997a).
An a MSB3 gic1-Δ1∷LEU2 gic2-Δ2∷TRP1 segregant from YEF1239 × CCY1042-12B was mated to CCY1042-12B.
Strain YEF1154 was transformed with the low-copy TRP1 GAL1-CDC24 plasmid pMGF5 (see Table 2), and a segregant containing both cdc24Δ∷HIS3 and pMGF5 was isolated on plates containing 2% glucose + 2% galactose.