Table 2.
Plasmids used in this study
| Plasmid | Characteristics | Reference or source |
|---|---|---|
| YEp13 | High-copy (2 μm), LEU2 | Rose and Broach, 1991 |
| YEp24 | High-copy (2 μm), URA3 | Rose and Broach, 1991 |
| YEp352 | High-copy (2 μm), URA3 | Hill et al., 1986 |
| YEplac181 | High-copy (2 μm), LEU2 | Gietz and Sugino, 1988 |
| YIplac211 | Integrative vector, URA3 | Gietz and Sugino, 1988 |
| pSM217 | High-copy (2 μm), URA3 | Chen et al., 1997b |
| pPB191 | MSB1 in YEp24 | Bender and Pringle, 1991 |
| YEp352-CLA4 | CLA4 in YEp352 | F. Cvrčkováa |
| pMGF5 | CDC24 under GAL1 promoter control in a low-copy (CEN), TRP1 vector | Miyamoto et al., 1991 |
| YEp352-CDC42 | CDC42 in YEp352 | See text |
| YEp13-MSB1 | Full-length MSB1 in YEp13 | See text |
| YEp13-CLA4* | A truncated CLA4 in YEp13 | See text |
| YEp352-42CLA4* | CDC42 and the truncated CLA4 in YEp352 | See text |
| YEp13-MSBX | A plasmid from the YEp13 genomic library that carries three ORFs | See text |
| YEplac-MSB3 | MSB3 from YEp13-MSBX subcloned into YEplac181 | See text |
| YEplac-MSB3D | A derivative of YEplac-MSB3 with an internal deletion in MSB3 | See text |
| YEplac-ORF1/2 | YNL295W and YNL294C from YEp13-MSBX subcloned into YEplac181 | See text |
| YEplac-MSB4 | PCR-amplified MSB4 cloned in YEplac181 | See text |
| YEplac-3HA-MSB3 | 3HA-MSB3 in YEplac181 | See text |
| YEplac-3HA-MSB4 | 3HA-MSB4 in YEplac181 | See text |
| pCC1107 | High-copy (2 μm), URA3, MSB3 | See text |
| pCC1108 | High-copy (2 μm), URA3, MSB4 | See text |
| pCC904 | High-copy (2 μm), URA3, GIC1 | See text |
| pCC967 | High-copy (2 μm), URA3, GIC2 | See text |
a
Derived from plasmids described by Cvrčkováet al. (1995).