Table 2.
A summary of the sensitivity/resistance of various mutant yeast strains to glucose removal at the translational level
| Relevant genotype | Resistance to translational inhibition on glucose withdrawala | Ratio of [35S]-methionine incorporation with or without glucoseb | Doubling time (min)c | Repressed (R) or derepressed (DR) on glucosed |
|---|---|---|---|---|
| Wild typee | − | >16 | 80–145e | R |
| snf1-Δ3 (yAS2575) | − | N | 105 | R |
| reg1Δ (yAS2576) | +++ | 2.29 | 110 | DR |
| reg1Δ snf1Δ (yAS2605) | − | N | 95 | R |
| hxk2Δ (yAS2578) | +++ | 1.89 | 100 | DR |
| hxk2Δ snf1Δ (yAS2606) | − | N | 240 | R |
| HXK2 hxk1Δ glk1Δ (yAS2504) | − | N | 85 | R |
| hxk2S15A hxk1Δ glk1Δ (yAS2505) | − | N | 85 | DR |
| glc7-T152K (yAS2574) | − | N | 100 | DR |
| glc7-Q48K (yAS2511) | + | 5.76 | 100 | DR |
| gsf1-1 (yAS2579) | − | N | 115 | DR |
| gsf2-Δ1 (yAS2580) | − | N | 85 | DR |
| mig1Δ (yAS2529) | − | N | 100 | DR |
| ssn6-Δ6 (yAS2498) | ++ | N | 215 | DR |
| rgt1Δ (yAS2527) | − | N | 100 | N |
| RGT2-1f (yAS2532/yAS2567) | − | N | 90/200f | N |
| SNF3-1 (yAS2566) | − | N | 200 | N |
| rgt2Δ (yAS2533) | − | N | 120 | N |
| snf3Δ (yAS2534) | − | N | 120 | N |
| snf3Δ rgt2Δ (yAS2537) | ++ | 2.15 | 225 | DR |
| snf3Δ rgt2Δ snf1Δ (yAS2602) | ++ | 2.81 | 230 | N |
| grr1Δ (yAS2577) | − | N | 110 | DR |
| tpk1w1 tpk2Δ tpk3Δ (yAS963) | +++ | 1.67 | 220 | R |
| tpk1Δ tpk2w1 tpk3Δ (yAS964) | +++ | 1.72 | 260 | R |
| tpk1Δ tpk2Δ tpk3w1 (yAS965) | − | N | 145 | R |
| tpk1Δ tpk2Δ tpk3Δ | ||||
| msn2Δ msn4Δ (yAS2599) | ++ | 1.94 | 139 | N |
| bcy1Δ (yAS956) | − | N | 90 | N |
| RAS2val19 (yAS982) | − | N | 135 | N |
| cdc35-11 (yAS2501) | −/+ | N | 155 | N |
| gpa2Δ (yAS2540) | − | N | 100 | N |
| gpr1Δ (yAS2541) | − | N | 100 | N |
| sch9Δ (yAS2542) | −/+ | N | 125 | N |
Cells were generally grown on YPD and then transferred to YPD or YP. It was noted that certain resistant strains grown on SCD gave lower levels of translational resistance to glucose withdrawal.
Rates of incorporation were measured after transfer to medium with or without glucose. N, not determined.
Approximate doubling time of the strain on YPD unless plasmid maintenance required the use of SCD-minus medium.
Measured by growth on YP sucrose plus 2-deoxyglucose or taken from previous work in a number of laboratories. N, the strains were not tested.
Wild-type backgrounds to all the strains used were tested.
The dominant RGT2-1 allele was tested either on a plasmid in a wild-type strain (grown on SCD) or in the RGT2-1 strain.