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Amplifcation of the nested 270-bp specific-PCR product on ethidium-bromide-stained agarose gel from 10 fg of T. evansi DNA. Lane MW: molecular weight marker (1 Kb ladder); Lane 1–7: T. evansi DNA at a concentration of 10 ng, 1.0 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, respectively.
