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. 2006 May 20;5:2. doi: 10.1186/1475-9292-5-2

Figure 6.

Figure 6

Detection of T. evansi DNA by PCR using four different primers. Lane MW: molecular weight marker (1 Kb ladder); Lane 1: amplification of the primary 810 bp-PCR product using the outer pair of primers (TE1 and TE2); Lane 2: amplification of the nested 270-bp PCR product using the internal pair of primers (TE3 and TE4); Lane 3: amplification of the semi-nested 490-bp PCR product using the pair of primers semi-nested 1 (TE1 and TE4); Lane 4: amplification of the semi-nested 590-bp PCR product using the pair of primers semi-nested 2 (TE3 and TE2). Lane 5: primers-free PCR amplification (negative control).