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Effect of mutating the Sp1 recognition sites on the activity of podocalyxin promoter. Transient transfections were performed into HEK293 cells. Results represent mean ± SEM from four independent experiments performed in duplicates. Promoter activity of each transfection was expressed as a ratio of luciferase/renilla activities. Data are expressed as percent of the maximal activity of the -210 construct *p < 0.05 vs. non Sp1-site-mutated construct.
