Figure 2.

Nuclear segregation in actin cytoskeleton mutants. Using the assay in Figure 1, we determined the frequency of improper nuclear segregation. To determine this value, the curve for improper nuclear segregation (Figure 1, ○) was integrated during a 90-min period centered on the peak of mitosis. The area under the curve of proper nuclear segregation (Figure 1, ▪) was calculated for the same 90-min period. The area for improper nuclear segregation was divided by the sum of the two areas (inset). Mutations were tested for genes whose products localize to actin cables, localize to cortical actin patches, localize to a cap in the bud tip, or have some other connection to the actin cytoskeleton. The error bars represent the SEM for repeated experiments. Wild type (wt), n = 7; arp1Δ, n = 5; sac6Δ, n = 2; sla1Δ, n = 2; and myo1Δ, n = 2.