Figure 5.

Orientation of astral microtubules before movement of the spindle into the bud neck. Cells were treated as described for Figure 4 and examined during the 3 min preceding movement of the spindle into the neck. Cells were scored for orientation of astral microtubules from the spindle pole body destined for the bud. Astral microtubules were seen to project only into the bud, both into the bud and into the mother, or only into the mother. All cells oriented at least one astral microtubule into the bud before moving into the bud neck. n = 33 for DMSO-treated cells and n = 29 for latrunculin (LatA)-treated cells.