Table 5.
VNTR locus-specific primers and the predicted sizes of amplicons from N. meningitidis strains Z2491, MC58 and FAM18.
| Locus | Primer designation | Primer sequence (5' → 3')a | Tm(°C) | Predicted size of amplicon (bp) | ||
| Z2491 | MC58 | FAM18 | ||||
| NMTR1 | NMTR-1 F | 6-FAM-GGGTCAAAAGACGGAAGTGA | 54.9 | 351 | 421 | 379 |
| NMTR-1 R | AAAATCATCCGAATCAATAAAGAC | 49.8 | ||||
| NMTR2 | NMTR-2 F | PET-GTGCGCCAGTAAGAAAATACAAT | 53.9 | 327 | 236 | 341 |
| NMTR-2 R | TCAGAAAAGTTTTGCATTTTGAA | 50.1 | ||||
| NMTR6 | NMTR-6 F | 6-FAM-GCGGCATCTTTCATTTTGTC | 52.8 | 165 | 165 | 207 |
| NMTR-6 R | CGAAGAAGCGAAAGACCAAG | 53.9 | ||||
| NMTR7 | NMTR-7 F | CCATCCTTATCCGAATCTGAA | 55 | 231 | 227 | 215 |
| NMTR-7 R | VIC-CTGAAACCCTGCCTGAAGAA | 53.4 | ||||
| NMTR9 | NMTR-9 F | PET-GGAAAGAATGATGAAAATCAAAGC | 51.3 | 243 | 225 | 396 |
| NMTR-9 R | CCGTCTGAAAAGCGGATACC | 55.8 | ||||
| NMTR9a | NMTR-9a F | GTTGTTGCCGACCAAGTTTT | 54.4 | 218 | 188 | 248 |
| NMTR-9a R | 6-FAM-GAACCTTGCAATGCGTTCAC | 55.2 | ||||
| NMTR9b | NMTR-9b F | CGACTTCATCGTCCACAAAA | 53 | 200 | 200 | 182 |
| NMTR-9b R | VIC-GGCTTTGTCTGCCTGTACG | 56.3 | ||||
| NMTR9c | NMTR-9c F | GGAAATCTGCGCTTTCGTAG | 54 | 202 | 187 | 202 |
| NMTR-9c R | NED-TCATGTCAGCAATTCCCTCA | 54 | ||||
| NMTR10 | NMTR-10 F | NED-GGCATCGATGATGTGAAACA | 53.3 | 221 | 251 | 221 |
| NMTR-10 R | GTGCTGAAGCACCAAGTGAA | 55.9 | ||||
| NMTR12 | NMTR-12 F | CAAAGAGAGAGTGGAAGAACATCA | 54.5 | 218 | 548 | 481 |
| NMTR-12 R | PET-AATGACGAAGAGTGGCAGGATT | 56.6 | ||||
| NMTR18 | NMTR-18 F | AACGGAAAATTCCTGCACAA | 53.1 | 182 | 182 | 209 |
| NMTR-18 R | VIC-CGTTTTCCGTGTTCCTGATT | 53.4 | ||||
| NMTR19 | NMTR-19 F | NED-GACATATTGTGCGATGTCGAG | 53.3 | 186 | 186 | 225 |
| NMTR-19 R | CGCCAACAGAAAAGAATACGA | 53.6 | ||||
a 6-FAM, VIC, NED and PET are ABI compatible dyes (Applied BioSystems, Foster City, CA, USA).