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. 2006 May 19;7:40. doi: 10.1186/1471-2202-7-40

Figure 2.

Figure 2

ADX increased TGF-β1 NA expression in the hippocampus. Photomicrographs show the changes in TGF-β1 mRNA levels in hippocampus of: (A) control (sham-operated), (B) 2 days after ADX (ADX-2), and (C) 5 days after ADX (ADX-5) animals. The in situ hybridisation was performed using a digoxigenin-labelled oligonucleotide probe complementary to TGF-β1 mRNA (524 pb – 563 bp; accession number NM_021578.1). The specificity of the hybridisation was evaluated using a 100-fold excess of unlabelled oligonucleotide (D). Scale bar = 500 μm.