Figure 2.

Hybridization of the 225-kb amplicon ends within a 2.6-kb restriction fragment of cosmid 79 that lies near an inverted repeat. (A) Cosmid 79 (Mizukami et al., 1993) was digested with EcoRI (E) or EcoRI plus XhoI (X), followed by gel electrophoresis. Left, ethidium bromide (EtBr) staining. Right, hybridization with labeled 225-kb amplicon. The vector bands exhibit weak hybridization with this probe. (B) Restriction map of cosmid 79. The fragments that hybridize with the 225-kb amplicon are shaded. The end point of the amplified DNA is within the 2.6-kb EcoRI fragment. PCR products W, X, and Y were used as probes to check the restriction map (our unpublished results).