Figure 5.

Hybridization of the 180-kb amplicon ends within the 3.2-kb BamHI–EcoRI restriction fragment of cosmid 1898. (A) Cosmid 1898 (Mizukami et al., 1993) was digested with EcoRI, and the fragments were separated by gel electrophoresis. Left, ethidium bromide–stained gel. Right, hybridization with labeled 180-kb amplicon. Only the 4.9-kb EcoRI restriction fragment hybridized. (B) Partial restriction map of cosmid 1898. Hybridization data from A are shown in relationship to the restriction map. The end point of the amplified DNA lies within the 4.9-kb EcoRI fragment. Additional data (our unpublished results) localize the end point to within the 3.2-kb BamHI–EcoRI fragment. E, EcoRI; B, BamHI.