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Phosphorylation of Wee1 by p42 MAPK results in a modest increase in kinase activity in vitro. Kinase activities are expressed in arbitrary units and shown as averages ± SD from three experiments. Open bars represent the activity of Wee1 in the presence of p42 MAPK or MEK R4F alone. The shaded bar shows the activity of Wee1 in the presence of both p42 MAPK and MEK R4F.
