Figure 5.

Mos potentiates the activity of Wee1 in cycling egg extracts. After immunodepleting extracts of endogenous Wee1, various amounts of recombinant Wee1 were added back in the presence (▪) or absence (□) of Mos protein. The Wee1-depleted extracts entered mitosis (assessed by nuclear envelope breakdown) 40 min after the initiation of cycling, 10 min before mock-depleted extracts (compare Figure 1). Data are expressed as the time of nuclear envelope breakdown in the Wee1-supplemented extracts relative to that of the Wee1-depleted extracts (“mitotic delay”). Data shown are from one (the 20 and 40 nM Wee1 data points) or two (the 0, 10, and 30 nM Wee1 data points) experiments. Error bars represent ranges.