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Transient activation of p42 MAPK during mitosis has no measurable effect on the length of the subsequent interphase. Cycling extracts were incubated with U0126 (100 μM; ▪) to prevent mitotic activation of MAP kinase or with an equal volume of DMSO (1%; □), and samples were removed periodically for histone H1 kinase assays. This concentration of U0126 fully blocked the mitotic activation of p42 MAPK (our unpublished results).
