Figure 9.

Galtase-GFP distribution in blastomeres arrested in prometaphase. (A) Interphase, 6 min after nuclei reformed after the sixth cleavage. The seawater in the chamber was changed to seawater containing 1 μM nocodazole 2 min 30 s after this image was taken. (B) Thirty-six minutes after nocodazole. To monitor the effect of nocodazole, the embryo was imaged at low magnification, low laser illumination at 30-s intervals. NEBD of the seventh cleavage occurred ∼11 min after nocodazole was added to the chamber. The blastomere was imaged with identical laser intensity and instrument settings as in A. The fluorescence pattern appears to be coming largely from the ER, which is brighter than in the interphase cells. There are some bright spots scattered throughout the cell, but they are much fewer and smaller than in interphase cells. Bar, 10 μm.