Figure 11.
In vitro phosphorylation of Gcn4 by ha-Pcl1–associated kinase activity. Different substrates (indicated at the bottom) were incubated with Protein A-agarose beads carrying Pcl1-associated kinase activity immunoprecipitated from various cell extracts. pho85Δ indicates that the extract was made from a pho85 mutant strain; all other extracts were from wild-type cells. starv. and CHX refer to the same treatments as in Figure 9. no Ab refers to a blank immunoprecipitation in the absence of antibody. The two arrowheads at the right indicate the two phosphorylation bands of the GST-Gcn462–202 substrate.