Figure 2.
In vitro cleavage of Cas by recombinant caspases. (A) 35S-Labeled rat Cas was generated by in vitro translation and incubated with bacterial cell lysates (20 μg) containing overexpressed recombinant caspases (caspase-1, -3, and -8). Cas was cleaved by caspase-3 and partially cleaved by caspase-1 but was unaffected by caspase-8. (B) Incubation of the in vitro–translated products with either DEVD-cmk or ZVAD-fmk, two membrane-permeant caspase antagonists, inhibited Cas cleavage in a concentration-dependent manner. Molecular mass standards are shown on the left.