Figure 8.

Interaction between Cas and paxillin in apoptotic cells. (A) Cell lysates were immunoblotted with Cas mAb or paxillin mAb. (B) Cas and paxillin were immunoprecipitated from control (C), adherent (H), or apoptotic (A) cells by Cas-2 Ab or paxillin mAb, and the interaction between Cas and paxillin was detected on immunoblots probed with Cas mAb or paxillin mAb. The partial cleaved Cas (>100-kDa band) binding to paxillin is indicated by the arrow on the right. The interaction between Cas and paxillin was dramatically decreased in adherent cells and absent in apoptotic cells. Molecular mass standards are shown on the left (arrowheads).