Figure 7.

Electron microscopic analysis reveals that prAPI is associated with membrane sac structures in the apg5^ts mutant. The apg5^ts strain was grown to log phase in YPD and shifted to 38°C for 3 h. Samples were prepared for electron microscopy by rapid freezing and freeze-substitution fixation and stained with lead citrate for 1 min (a–c) or stained with lead citrate for 30 s and immunostained with anti-API antibodies (d), as described in MATERIALS AND METHODS. The arrows mark membranous structures around the Cvt complex. V, vacuole.