Figure 5.

Soluble pool of Eno2p is required for tRK1 import. (A) Effect of antibodies on tRK1 mitochondrial import. The antibodies used are indicated at the top. As 1× concentration, we used the concentration of antibodies with the same immunological capacity (determined by Western analysis). The left panel corresponds to tRK1 import into antibody pretreated mitochondria; the right panel corresponds to tRK1 import directed by immunodepleted IDP. (B) tRK1 import assays directed by IDP isolated from yeast strains indicated at the first line on the top (see explanations below the panel) into mitochondria isolated from different strains (second line). (Bottom) Efficiency of tRK1 import. (+Eno2p) Addition of 0.1 μg of recombinant Eno2p to the IDP of the strain indicated.