Figure 6.

Translation of MSK1 mRNA is compartmentalized. Quantification results obtained after RT–qPCR measurement of mRNAs coding for Atp1 (first subunit of the mitochondrial ATP synthase), Krs1 (cytoplasmic lysyl–tRNA synthetase), Msk1 (preMsk1p), and Eno1/2 (enolase 1 and 2; oligonucleotide primers used do not distinguish between the two mRNAs). To obtain the indicated values, the absolute amounts of mRNAs were first calculated using purified T7-transcripts corresponding to each analyzed mRNA, then the ratio between each mRNA amount in the mitochondrially associated RNA pool and in the total RNA was calculated. In the control experiment, the isolation of mitochondria for RNA purification was performed in the absence of cycloheximide (−CYH).