Fig. 2.

GFP-mPNGase was coimmunoprecipitated with mAMFR-myc in COS1 cells. COS1 cells were cotransfected with plasmids encoding GFP and mAMFR-myc (lanes 2 and 4) and GFP-mPNGase and mAMFR-myc (lanes 1 and 3). Cell lysates were subject to immunoprecipitation (IP) with monoclonal anti-Myc Ab (lanes 3 and 4) in RIPA buffer. The monoclonal anti-myc Ab and bound proteins were recovered with agarose beads containing coupled protein A. The cell lysate (lanes 1 and 2) and the proteins coupled to the protein G beads (lanes 3 and 4) were subjected to SDS/PAGE and immunoblotted (IB) with polyclonal anti-GFP (Upper) or anti-myc (Lower).