Fig. 3.
Requirement of RIC1/F1α for import of type I and II tRNAs. (A) Regulation of import by type I and II effector tRNAs. High specific activity import substrates (5 nM) were incubated in the absence or presence of low specific activity tRNATyr or tRNAIle effector (0.5 nM) and assayed for import into RIC-reconstituted proteoliposomes. (B) Import reconstitution assay. Mitochondrial extract from uninduced (WT) or 24-h tet-induced (KD) pGET(AS)RIC1-transformed cells was incubated with or without recombinant RIC1/F1α or with BSA (BSA), in presence of liposomes, which were then assayed for import of tRNATyr. (C) Import of the indicated tRNAs (5 nM) in absence or presence of low specific activity tRNATyr effector (0.5 nM), into liposomes reconstituted with knockdown extracts in the absence or presence of recombinant RIC1/F1α.