Fig. 5.

Relationship between the RNA import complex and respiratory complexes of Leishmania. (A) L. tropica mitochondrial protein (200 μg) run in 6% BN PAGE along with molecular weight markers (M). (B) The largest BN PAGE band was excised from BN gel, denatured and run on SDS/12% PAGE along with affinity purified RIC (AF). RIC band numbers are indicated at right. (C) Immunoblot of mitochondrial complexes probed with anti-RIC1/F1α or F1β antibody. (D) Second-dimension analysis of RIC and complex V, showing Coomassie-stained subunits (Left), anti-RIC1/F1α immunoblot (Center), and Northwestern blot probed with ³²P-labeled tRNA^Tyr (Right). (E) (Left) Leishmania and human mitochondrial complexes resolved by BN PAGE. (Right) Northwestern blot probed with ³²P-labeled tRNA^Tyr. (F) Dodecyl maltoside extracted mitochondrial complexes were incubated with ³²P- and BrdU-labeled tRNA^Tyr followed by UV-cross-linking, BN PAGE, transfer to nitrocellulose membrane, and autoradiography. Coomassie-stained complexes are shown at left.