Fig. 4.
A cartoon of the folding process for tether points 11 (a) and 34 (b) at their respective folding temperatures. For tether point 11, U/I is designated as a starting point for the folding process to indicate that at Tf, the protein proceeds to its native state from a compact intermediate state. In contrast, when tethered at residue 34, the protein does not fold through an intermediate. The difference in the TS for these two tether points is also illustrated. Although tether point 11 has a compact, bulk-like TS with a well formed core, tether point 34 folds through a highly unstructured TS that is significantly different from that of the bulk.