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. 2006 May 22;103(22):8447–8452. doi: 10.1073/pnas.0601040103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 1. — RTEs remain readily detectable even in 2-year-old mice, and thymic output as a function of thymic size is relatively age-independent. Splenocytes and thymocytes from individual RAG2p-GFP Tg mice of the indicated ages were stained for CD4 and CD8 surface expression. (A) GFP^hi and GFP^low gates were defined on SP thymocytes (Left) and applied to splenocytes (Right). (B) The number of total and DP thymocytes was determined as a function of age. (C) The percentages of GFP^hi (filled squares) and GFP^low (open circles) cells among CD4⁺ and CD8⁺ splenocytes were determined in mice of the indicated ages. (D) By using organ cell counts and the percentage of DP thymocytes and RTEs (GFP^hi plus GFP^low), the total number (Left) and the number per 100 DP thymocytes (Right) of splenic CD4⁺ (open squares) and CD8⁺ (filled circles) RTEs were calculated.