Table 1.
Summary of phenotypic properties of VSV L gene mutants
| Mutant | Plaque size, mm | Titer, 24 h, log10 pfu/ml | RNA synthesis |
Protein | 7mG% [AdoMet] |
GpppAm, % | ||
|---|---|---|---|---|---|---|---|---|
| Cells | In vitro | 1 mM | 0.2 mM | |||||
| rVSV | 4.1 ± 0.5* | 9.7 ± 0.2 | 100 | 100 | 100 | 97 | 94 | 2 |
| G1670A | 2.8 ± 0.4† | 8.8 ± 0.1 | 108 | 90 | 80 | 20 | <1 | 75 |
| G1672A | 2.2 ± 0.3† | 8.7 ± 0.2 | 110 | 100 | 90 | 10 | <1 | 80 |
| G1674A | 4.2 ± 0.6* | 9.8 ± 0.1 | 110 | 110 | 105 | 92 | 36 | 5 |
| G1675A | 1.5 ± 0.3† | 7.5 ± 0.2 | 50–250§ | 50 | 75 | 15 | <1 | <1 |
| G4A | 1.4 ± 0.3‡ | 6.1 ± 0.4 | 55–350§ | 40 | 55–110¶ | <1 | <1 | <1 |
| D1735A | 3.0 ± 0.4† | 8.6 ± 0.2 | 70 | 55 | 40–90¶ | 30 | 10 | 15 |
| D1671V | 1.8 ± 0.3† | 6.8 ± 0.1 | 70 | 75 | 85 | <1 | <1 | <1 |
| S1673A | 3.1 ± 0.4† | 8.4 ± 0.1 | 70 | 60 | 35–90¶ | 20 | 10 | 75 |
| G4AD | 1.6 ± 0.3‡ | 6.4 ± 0.1 | 75–370§ | 35 | 80–110¶ | <1 | <1 | <1 |
pfu, plaque-forming units.
*Plaque diameter was measured at 24 h after inoculation.
†Plaque diameter was measured at 48 h after inoculation.
‡Plaque diameter was measured at 96 h after inoculation.
§Percentage of RNA varied as follows: P/M (50%)-V (250%); P/M (55%)-V (350%); P/M (75%)-V (370%).
¶Percentage of protein varied as follows: L (55%)-N (110%); L (40%)-N (90%); L (35%)-N (90%); L (80%)-N (110%).