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Rev1 is cell cycle-regulated and expressed maximally at G2/M phase. (A) Immunoblot against the protein A epitope shows Rev1 and Rad30 protein levels at indicated time points after release from G1 α-factor arrest. PGK (phosphoglycerate kinase) was used as a loading control. (B) FACS analysis of the DNA content of cells. (C) RT-PCR showing REV1 mRNA levels. ACT1 was used as a loading control.
