Fig. 2.

Rev1 protein is stable in both metaphase and telophase arrested cells. (A and B) Immunoblot showing Rev1-ProA and Pds1-HA in cdc23-1 (A) or cdc15-2 (B) arrested cells. Time points were taken every 20 min after release from α-factor and shift to the restrictive temperature. (C) Immunoblot showing Rev1 and Pds1 levels at the indicated times after release from the cdc15-2 block. Cells released from a cdc15-2 arrest fail to separate because of a cytokinesis defect and generate a 4C peak on FACS that is indicative of a second round of DNA replication.